The web server can be used for small-scale data sets (at most 100 substitutions, with no input sequence of length > 35,000 residues). Currently, this web site provides MutPred2.0. It requires protein sequences in the FASTA format, a list of amino acid substitutions in the corresponding FASTA headers (separated by spaces only), and a valid email address. The protein ID cannot contain spaces, semi-colons or commas.
The standalone executable can be used for genome-scale data sets. In addition to the standard MutPred2 input format (see above), the MutPred2 software also supports the output file from ANNOVAR's coding_change.pl program. This enables the straightforward movement between VCF files and MutPred2. To install and run MutPred2, you will need about 50 GB of hard disk space and about 8 GB RAM. Click on a link below to download.
Before downloading: please take some time to read the MutPred2 license agreement.